Mutations at Two Invariant Nucleotides in the 3′-Minor Domain of Escherichia coli 16 S rRNA Affecting Translational Initiation and Initiation Factor 3 Function
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چکیده
منابع مشابه
Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits.
Initiation factor-3 has been photochemically cross-linked to Escherichia coli 30 S ribosomal subunits by means of near-ultraviolet (> 285 nm) irradiation. The cross-linking was judged to be specific since noncovalent binding was required for subsequent cross-linking and was prevented by the presence of 0.5 M NH4Cl or 0.5 mM aurintricarboxylic acid. Covalent attachment reached its maximum level ...
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Translational initiation factor-3 (IF-3) was characterized by 1H NMR spectroscopy as a function of pH and temperature and following chemical modifications. Spin-lattice relaxation times for individual resonances and bands were also measured. Several resonances were assigned to different amino acid residues by different criteria. Among these are the CH3-N of the N-terminal methionine which appea...
متن کاملEffect of initiation factor 3 binding on the 30S ribosomal subunits of Escherichia coli.
Under certain conditions, initiation factor 3 (IF-3) can cause the release of aminoacyl-tRNA bound to 30S ribosomal subunits of E. coli. It is shown that this IF-3-induced aminoacyl-tRNA release cannot be attributed to either nucleolytic attack or competition between IF-3 and aminoacyl-tRNA for the same ribosomal binding site. It was found that the 30S-aminoacyl-tRNA-codon complexes formed in t...
متن کاملEscherichia coli translational initiation factor IF3: a unique case of translational regulation.
The Escherichia coli translational initiation factor IF3 is encoded by an mRNA that has an unusual ribosome binding site. We have explored a mechanism that may account for the translation of IF3 and that provides regulation of the quantity of IF3 relative to ribosomes.
متن کاملPlant initiation factor 3 subunit composition resembles mammalian initiation factor 3 and has a novel subunit.
Eukaryotic initiation factor 3 (eIF3) is a multisubunit complex that is required for binding of mRNA to 40 S ribosomal subunits, stabilization of ternary complex binding to 40 S subunits, and dissociation of 40 and 60 S subunits. These functions and the complex nature of eIF3 suggest multiple interactions with many components of the translational machinery. Recently, the subunits of mammalian a...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1074/jbc.271.9.4693